5th World Congress on Virology
University of Kordofan,Sudan
Title: Molecular Studies on Tomato Leaf Curl Virus (TLCV) in Bara, Sudan
Biography: Sana Khalifa Mukhtar Badawi
Tomato samples with typical symptoms of Tomato Yellow leaf curl Disease (TYLCD) were collected open field and green house from Bara town (North Kordofan State) in Sudan. PCR was used to identify the pathogen using degenerate primers of Geminiviruses (AVcore and ACcore). To determine exactly the virus strain, multiplex PCR was used with sets of specific primers for tomato yellow leaf curl virus Almeria strain (TYLCV-Alm), tomato yellow leaf curl virus Israel strain (TYLCV-IL), tomato yellow leaf curl mild strain (TYLCV-mld) and tomato yellow leaf curl virus Sardinia strain (TYLCSV). All samples were positive with degenerate primers and negative with all other specific primers. PCR products were cloned and sequenced and the results showed that all isolates tested were found to be related to Tomato leaf curl Sudan virus. The highest nucleotide identities (>95%) were obtained with members of the species from Sudan and Yemen.
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