5th World Congress on Virology
University of Brasilia,Brazil
Title: HIV-1 production is dependent on the incorporation of Dynamin-2 by Nef
Biography: Thatiane L. Sampaio
Nef is a virulence factor for Human Immunodeficiency virus type 1 (HIV-1) and promotes progression to AIDS. Nef interaction with Dynamin 2 is required for HIV-1 infectivity, and Dynamin-2 is incorporated into HIV-1 particles. The objective of this study was to investigate the effects of the interaction between Dynamin-2 and Nef during HIV-1 production. Dyanmin-2 incorporation is necessary for both, HIV-1 wild type and Nef-deficient infectivity during depletion of Dynamin-2 by small interfering RNA. The overexpression of Dynamin-2 allows for the enhancement of infectivity of HIV-1 wild type, but is not able to rescue HIV-1 Nef-deficient infectivity. Moreover Dynamin-2´s overexpression inhibits HIV-1 Nef-deficient production. A Dynamin-2 product is detected less in HIV-1 wild type particles when compared to Nef-deficient HIV-1 particles. This data suggests that Nef and Dynamin-2 interaction is an important factor for virus infectivity and production.
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