Jun-Wen Li
Institute of Health and Environmental Medicine,China
Title: Full length sequence analysis of Hepatitis E virus isolates: showing potential determinants of virus genotype and identity
Biography
Biography: Jun-Wen Li
Abstract
Hepatitis E virus (HEV) is an important public health problem in the developing world. The complete genome sequence of a genotype 4 strain of HEV(CH-YT-HEV02) from a patient has been determined. Phylogenetic analysis showed that CH-YT-HEV02 belongs to genotype 4, subtype 4a. Judging from the phylogenetic tree based on the full-length nucleotide sequences of all 70 genotype 4 HEV isolates retrieved from GenBank, the CH-YT-HEV02 isolates could serve as an indigenous strain. A broader comparison of amino acid within the HVR of different HEV isolates showed that the variety of HEV strain involved in different amino acid sequence of HVR, so the HVR region can be served as the identity of a HEV strain. Moreover, there were some special amino acid substitutions in the HVR region of different HEV genotypes. These conserved amino acids may proved to be useful diagnostic indicator of genotype and species of origin. These results could shed light on that genotypes 1 and 2 strains are restricted to humans whereas genotypes 3 and 4 are zoonotic. In addition, Our data comparing all HEV genotypes identified two amino acid motifs within ORF2(80-83 aa) and ORF3 (66-73 aa), which may prove to be a useful diagnostic indicator of genotype of an isolate. The two amino acid motifs in ORF2 and ORF3 might serve as signatures of genotype diversity of HEV, and the results may facilitate to understand the classification and epidemiology of HEV isolates.