Biography
Biography: Eybishtz Assaf
Abstract
Tomato yellow leaf curl virus (TYLCV) is a whitefly-transmitted geminivirus which causes devastating loss of tomato crops worldwide. This study is based on the known resistance alleles Ty-1, Ty-3 which mapped to different region on chromosome 6. Recent publication suggests they rather be alleles of the same gene (Verlaan et.al; 2013). we collaborated with Dr. Rotem Neta from the Hebrew University and DYN Diagnostics LTD aimed to identify Real Time PCR (RT-PCR) based marker linked to gene confer resistance, a new RT-PCR markers, TY-13 found more reliable in our tested lines. We tested plants from 9 Tomatech commercial hybrids and 34 different lines from F-3 till F-10 generation lines. TY1 match in 72.22 %, TY3 match in 77.77% and TY13 match in 81.11% to the symptom scoring. The added advantage was while TY1 and TY3 markers mismatch was false positive and negative, TY13 mismatch was only false positive, meaning no sensitive plant escaped from TY13 marker. Further test was made on 376 plants, from 4 different commercial hybrids and 11 different F2 populations. In this analysis we identified four performances: full match between Ty1-Ty3-Ty13, match only between Ty1- Ty13, match only between Ty3- Ty13 and incompatibility between Ty1, Ty3, Ty13. The correlation between the markers was 80% for TY1, 73% for TY3 and 90% for TY13. TY3 marker didn’t respond on one of our F2 populations from the S. habrochaites source, while TY13 fits to TY1 and clearly identified with the symptom scoring. These results confirmed the reliability and the advantage of using TY13 marker. TY13 identify resistance from several sources, reduces the need of using two different markers in parallel, and increasing the reliability of the decision in the breeding process.