Kavous Ayazpour
Islamic Azad University,Iran
Title: Full Sequencing of an isolate of Tomato Yellow Leaf Curl Virus from Iran and study of its phylogenetic relationship
Biography
Biography: Kavous Ayazpour
Abstract
For full genome sequencing and determination of taxonomic position of tomato yellow leaf curl virus isolated from the Aabdan, Kangan and Asalooyeh of Boushehr province, 28 samples of tomato was collected from tomato farms of these regions in the winter of 1391. Using degenerate primers of begomoviruses (primer BC/ PCRV 181) a fragment (500bp) was amplified in Asalooyeh sample in polymerase chain reaction (PCR). Using the sequence of this fragment, a primer pair was designed (FarsC/FarsV) to achieve the full genome sequence of the virus. Using the combination of primers (FarsC/ PAL1V 1978) / (FarsV /TYLCV-[Ab] 1997C) complete sequence of the Asalooyeh virus was determined. Using Bioinformatics programs and the comparison of complete genome of the virus with other viruses in Gene bank the taxonomic position of Asalooyeh isolate was determined. This is a member of Omani group of tomato yellow leaf curl virus. Also according to the obtained dendrogeram and proximity of this isolates with Arad isolate of Larestan shows the possible movement of virus between border regions of Fars and Boushehr provinces.