Canadian Food Inspection Agency, Canada
Title: Generation of monoclonal antibodies against foot-and-mouth disease virus SAT 2 and development of lateral flow strip test for virus detection
Biography: Ming Yang
Foot-and-mouth disease (FMD) remains one of the world’s most widespread epizootic and highly contagious animal diseases affecting a wide host range species. More than 100 countries worldwide are not yet accepted as FMD free by the World Organisation for Animal Health. FMD virus (FMDV) is recognized as seven serotypes: O, A, C, Asia 1, SAT 1, SAT 2 and SAT 3. Several FMD outbreaks due to SAT 2 had been reported from 1990 to 2012. The development of a rapid and easily performed test for FMD detection is critical for controlling FMD outbreaks and containing its spread. The aim of the project was to generate FMDV/SAT2 specific monoclonal antibodies (mAbs) and develop a lateral flow immuno chromatographic (LFI) strip test for the rapid detection of FMDV/SAT 2. A total of eight mAbs were generated and examined for their reactivity and specificity using ELISAs. The mAb #10 was selected as the capture mAb because it reacted with all tested SAT 2 isolates. The LFI strip test was developed using two mAbs. The LFI strip test was able to identify SAT 2 isolates (n=23) in culture supernatants. The calculated diagnostic specificities were 100% and 98% for the strip test and ELISA, respectively. Thirty four of 50 FMDV/SAT 2 PCR-positive tissue suspensions from experimental inoculated animals without application were identified as positive by the LFI strip test. While, sixteen samples were positive using an ELISA. Diagnostic sensitivity for LFI strip test and ELISA were 67% and 33%, respectively calculated based on the fifty samples. In conclusion, a lateral flow strip test for detection of FMDV/SAT 2 was developed. The performance of the strip test in terms diagnostic specificity and sensitivity was higher than the ELISA. The ability of strip tests to generate rapid results would be useful for the early diagnosis on-site during FMD outbreaks.