9^th International Virology Congress and Expo

Biography

Biography: M Bahanur Rahman

Abstract

Total 15 samples were collected from cattle infected with FMD, of which 85 (56.29%) was adapted in BHK-21 cell. Viral RNA was extracted from virus infected BH-21 cell culture fluid using RNA extraction kit (Promega^®, USA) and used for amplification of VP1 gene with specific primers by RT-PCR for FMD virus serotyping, of which, 31 (43.67%), 26 (36.62%), 10 (14.08%) were positive for serotype A, O, Asia 1, respectively and 4 (5.63%) for mixed. Partial sequencing of VP1 gene was performed two from each serotypes and comparison conducted using the BLAST search and 92-99%, 92-100% and 96-98% homology were found with some FMD virus serotypes O, A and Asia-1 isolates of Bangladesh, India, Pakistan, Nepal and Bhutan, respectively. Isolates of this study belonged to PanAsia-02 sub-lineage of ME-SA topotype (serotype O), genotype VII (18) of ASIA topotype (serotype A) and Lineage C (serotype Asia-1), respectively.  BAU FMD Vac-1 and -2 developed from isolated FMD virus and antibody titers were determined in sero-negative calves by ELISA and SNT and compared with commercially available FMD vaccines. Vaccines were administered in single and booster dose order; of which BAU FMD Vac-1 produced better immune response than other vaccines including BAU FMD Vac-2. Highest antibody titers were found in all vaccines at 60 dpv, and after 60 dpv the antibody titers gradually decline. Protective immunity persists up to 5 months (single dose) and 6 months (booster dose) in case of BAU FMD Vac-1. On the other hand in case of with BAU FMD Vac-2, Raksha® and Aftovaxpur® protective immunity persisted only 4 months (single dose) and 5 months (booster dose). Efficacy test of BAU FMD Vac-1 and 2 vaccines were carried out in guinea pigs and found 100% potent against FMD virus serotypes O, A and Asia-1.