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6th Euro Virology Congress and Expo

Madrid, Spain

Yolanda Revilla

Yolanda Revilla

Centro de Biología Molecular “Severo Ochoa”, Spain

Title: Monocyte Porcine Cell lines for Productive African Swine Fever Virus Infection

Biography

Biography: Yolanda Revilla

Abstract

ASFV is highly pathogenic double-stranded DNA virus with a marked tropism for cells of the monocyte-macrophage lineage. Although monkey cell lines such as Vero or COS allow the adaptation of ASFV strains after several passages, a suitable porcine cell line able to efficiently support ASFV infection is necessary to develop models for cell-host interaction and vaccine studies. For this purpose, four different porcine cell lines from monocyte-macrophage origin (IPAM WT, IPAM-CD163, CΔ2+ and WSL) have been tested in order to set up the most similar conditions to the infection in primary alveolar macrophages (PAM) in terms of phenotype, ASFV infection susceptibility and viral production. To achieve this, we analyzed on these lines the presence of CD163 and CD169 cellular surface receptors since they are linked to differentiation and maturation of the macrophages and seem to be closely related to ASFV infection. ASFV susceptibility was analyzed in the infected cells by the expression of the viral late protein p72 and viral production by titration on plaque assays. Results showed that although all porcine cell lines analyzed were susceptible to ASFV infection, none of them was as efficient as PAM in terms of virus production. Future experiments will be focus on describing which cellular factors are related with the ability of porcine cell lines to support an ASFV productive infection in order to establish a suitable model of study.