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6th Euro Virology Congress and Expo

Madrid, Spain

Beti Ernawati Dewi

Beti Ernawati Dewi

Universitas Indonesia, Indonesia

Title: Development of animal model for dengue virus antiviral trial using infected cells

Biography

Biography: Beti Ernawati Dewi

Abstract

Dengue virus (DENV) infection is still a serious health problem in Indonesia with no specific antiviral to treat the patients. Due to the lack of a good animal model, the development of antiviral has not been completely elucidated, In this study we develop a mice model of DENV infection for antiviral experiment. DDY mice were injected with 5.5 x 106 DENV-2 New Guinea C infected Huh-7 and Vero cell via intra peritoneal. The presence of DENV in plasma sera were evaluated by focus assay at 6, 24 and 48 hours post infection. In mice with DENV-2 infected Vero cells, the titer of DENV-2 was 1.7x102 FFU/ml at 6 hours post infection. At 24 and 48 post infection no DENV were found in plasma mice with DENV-2 infected Vero cells. The higher titer was found in mice with DENV-2 infected Huh-7 cells with 5.8 X 103FFU/ml and 3,6X103 FFU/ml at 6 hours and 24 hours, respectively. The DENV viremia results demonstrate that DDY mice injected with DENV infected Huh-7 are a promising simple animal model for DENV antiviral trials in future. Keywords: Animal Model, Dengue virus, Vero cell, Huh-7 cells

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