Serological Methods In Virology

A serological diagnosis is prepared by detecting the rising titres of antibody or detection of IgM. By serological methods most of the common viral infections are diagonized. Western blot, Radioimmunoassay,Enzyme linked immunosorbet assay,Particle agglutination, Recombinant immunoblot assay are the newer techniques of serology. It is the main stay of viral diagnosis. Complement Fixation test, Virus Isolation,Electron microscopy, Haemagluttination inhibition test, Enzyme Linked Immuno Assay, IgG Antibody avidity ,Single radial haemolysis, ,Neutralization, , Immunoflurorescence Molecular methods are the commonly used viral serological methods used in the field of virology. ELISA was developed in 1970. Competitive,Sandwich and Antibody capture methods are the assay principles used in ElISA technique. In Haemagluttination inhibition test the main moto is to prevent haemogluttination caused by antibodies which goes against viral proteins.

  • RIA and Immunosorbet Assay
  • Elisa,Haemagluttination Inhibition Test,Neutralization,Complement Fixation Test
  • Limitations of Serological Diagnosis
  • Viral Genome Detection
  • Electron Microscopy and Antigen Detection
  • Virus Isolation, Single Radial Haemolysis

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